Endotoxin-induced serum factor kills malarial parasites in vitro

Differential sensitivity in vivo of lethal and nonlethal malarial parasites to endotoxin-induced serum factor.

Sera from mice infected with Plasmodium yoelii or Plasmodium berghei and given endotoxin contained nonspecific mediators which killed both species of parasite and tumor cells in vitro. The sera resembled tumor necrosis sera obtained from mice given macrophage-activating agents such as Propionibacterium acnes (formerly designated Corynebacterium acnes) or Mycobacterium bovis BCG and then endotox...

Malarial Fevers and Malarial Parasites

An endotoxin-induced serum factor that causes necrosis of tumors.

In studying "hemorrhagic necrosis" of tumors produced by endotoxin, it was found that the serum of bacillus Calmette--Guerin (BCG)-infected mice treated with endotoxin contains a substance (tumor necrosis factor; TNF) which mimics the tumor necrotic action of endotoxin itself. TNF-positive serum is as effective as endotoxin itself in causing necrosis of the sarcoma Meth A and other transplanted...

Studies on Malarial Parasites

1. Methods have been described for the preparation and sterilization of a synthetic nutrient medium which supports normal growth during one 24 hour asexual cycle of the erythrocytic form of P. knowlesi. 2. Successive subcultures with good multiplication can be carried out when whole blood or plasma is added to the medium. Data are presented from two such experiments where the sixth and seventh ...

584 Studies on Malarial Parasites

When Laveran (1) discovered living malarial parasites over 65 years ago in the blood of patients with certain fevers, a new era in the study of malaria was initiated. This discovery of malarial organisms and the subsequent experimental proof by a series of workers that they were the etiological agents of the disease failed, however, to lead to the immediate successful cultivation of the parasit...